One of the promising methods to improve (stabilization) the visual functions authors consider the use of artificial fluorophores, in particular, quantum dots (QDs ), as well as combinations of subretinal implant and QDs. In this paper we experimentally studied the physicochemical properties of the InP/ZnS QDs and their sterilization using track membranes for subsequent use in vitreoretinal surgery. The temperature depending of photoluminescence (PL) of the quantum dots in saline, are investigated. The spectrum of absorption and emission are stable in the temperature range from 22 ºС to 50 ºС, which points to the stability of the photodynamic characteristics of QDs in terms of the temperature homeostasis of the human body. An analyze of photodynamic properties the QDs with immunocompetent environment of saline with antibodies to influenza virus H1N1, are processed. We found that the presence of a foreign protein can change the photodynamic properties of QDs and negatively affect the process of the photo transduction. The absorption spectra and emission QDs with biological tissues eyes, in particular, with biopsy specimens of the retina, subretinal fluid and vitreous humor, are investigated. Established a negligible impact anatomical substrate eyeball to photodynamic properties of the QDs. To visualize the conformational structure of the solution of QDs and analyze the conglomerates of QDs, comparing the size range of QDs with passports that they, as well as measuring the distance between the QDs for the occurrence of FRET - effect, we used the method of atomic force microscopy. The average diameter of QDs, the distance between adjacent QDs amounted to 25-40 nm, which is sufficient to cause FRET- effect. We used a method of sterilization the QDs by means of track membranes with a pores diameter 42,3 microns. The results showed no bacterial growth. The physicochemical properties of QDs indicate a good promising of their using for degenerative retinal diseases.
|Translated title of the contribution||A study of physicochemical properties of quantum dots InP/ZnS for ophthalmology|
|Publication status||Published - 2014|
Level of Research Output
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