This work contains 52 pages of typewritten text, including 19 figures, 1 table and 106 literature sources. The work is devoted to the study of the diversity of the totality of the traits of the roots of four families of monocotyledonous plants – Orchidaceae Juss., Iridaceae L., Poaceae Barnhart and Cyperaceae Juss., in the conditions of the boreal climatic zone. The analysis included materials provided by the scientific advisor (data on the structure of roots of representatives of the families Orchidaceae, Poaceae, Cyperaceae) and original collections. The goal is to identify the diversity of root trait syndromes in four families of monocotyledonous plants in the boreal zone. To achieve the goal, the following tasks were set: to study the structure of absorbing roots of representatives of the families Orchidaceae, Iridaceae, Poaceae, Cyperaceae; to study the external signs of root systems of representatives of the families Orchidaceae, Iridaceae, Poaceae, Cyperaceae; to compare the complex of quantitative and qualitative characters on the scale of the entire root system in taxa with different types of mycorrhizal, as well as with a predominance of nonmycorrhizal species. Using classical methods of structural botany, the external signs of root systems and the structure of absorbing roots were studied. Received microtome sections of absorbing roots, which were analyzed under a Leica DM 5000 microscope in a bright field and in blue beams with a wavelength of 365 nm (fluorescence microscopy). Using an eyepiece micrometer, the following was measured: the diameter of the root and stele, the thickness of the cortex, the length of root hairs, and the number of xylem vessels was counted. On longitudinal preparations, the occurrence of root hairs was determined. The number of orders of branching of the root system was fixed according to the functional classification [McCormack et al., 2015]. Mycorrhiza was determined by light microscopy (Leica DM 5000; × 100) after preliminary maceration of the roots in KOH and staining with aniline blue [Selivanov, 1981]. The data were processed using the EXCEL program, and the one-way ANOVA was carried out using the STATISTICA program. The research results showed that the roots of different monocotyledonous taxa differ greatly in their external and internal structure. Syndromes of root signs were identified: Orchidaceae – thick non-branching roots of with highly developed specialized mycorrhiza, with relatively thick bark and stele, long root hairs and without pronounced exoderm; Iridaceae – thick, highly branched roots of with powerful exoderm, short rare root hairs and intense AM; Poaceae – thin branched roots of with an indistinct exoderm, with short, frequently occurring root hairs and intense AM; Cyperaceae – thin, highly branched roots of with well-developed root hairs and other adaptations to autonomous soil nutrition, AM is rare.
|Date of Award||2021|
|Supervisor||Анна Анатольевна Бетехтина (Supervisor)|
Разнообразие морфологических и структурных признаков поглощающих корней четырех семейств бореальных однодольных: магистерская диссертация
Анянова, Ю. А. (Author). 2021
Student thesis: Master's Thesis