Развитие динамической спекл-интерферометрии для изучения свойств культивированных клеток как материала биомолекулярной электроники
: магистерская диссертация

Tesis doctoral: Master's Thesis

Resumen

An unresolved problem in research in the field of bioelectronics is the connection between the electrical signals coming from different parts of the cell, and the processes occurring within cells.
In this regard, the aim of this work was the development of the method of dynamic speckle interferometry, which allows to determine the parameters characterizing the physical processes in different parts of a single cell.
Object of research is living L-41 cell culture in the form of defrosted cells precipitated on a glass substrate.
Literature review on the use of cell cultures in the bioelectronic field, methods cell culture studies conducted. Optical properties of cells are shown. The theory of the method of averaging in dynamic speckle interferometry is briefly considered.
Speckle interferometric installation allowing at high optical magnifications to analyze intracellular processes assembled, adjusted and tested. Attestation of samples carried by optical microscopy. Dependences of the time average digital value of the intensity from pixel number and the time inside the cells are obtained. Dependences of the correlation coefficient of speckle imaging from time for different parts of the cell found.
It is shown that the method allows to detect the difference in the processes occurring in the nutrient solution (outside cells) and in cells and also in different parts of the cell.
Fecha de adjudicación2015
Idioma originalRussian
Institución adjudicataria
  • Ural Federal University
SupervisorАлександр Петрович Владимиров (Supervisor)

Citar esto

'