The object of the study was glucose. The relevance of the work lies in the practical application of enzyme-free electrochemical sensors to control blood glucose levels in diabetes mellitus. Objective: to develop an enzyme-free electrochemical sensor for glucose determination using organic nickel (II) complexes, as sensitive elements, and self-assembled polymer layers with molecular prints based on polyethyleneimine, as a selective element. The nickel (II) acetylacetonate complex exhibits electrocatalytic properties. Using the obtained sensor, glucose was measured in model solutions simulating blood serum. It was revealed the absence of the influence of extraneous compounds. The resulting MIPs have selectivity.