Исследование антирадикальной активности природных и синтетических антиоксидантов методом ЭПР-спектроскопии
: магистерская диссертация

学生论文: Master's Thesis

摘要

Substances with anti-radical activity (ARA) play a leading role in the body's defense system against free radicals. General disadvantages of the known methods of evaluating the APA expressed in the fact that measurements are carried out by indirect methods, making it difficult to quantify the content of substances with antiradical properties. The measurement results are presented in relative units, as reference substances are most often used: Trolox, ascorbic and gallic acid, exhibit different ARA, which does not allow to compare the results with each other. Thus, there is a need to develop direct, reference-free method of evaluating anti-radical activity. One possible solution to this potential problem is the method of EPR model 2,2-diphenyl-1-picrylhydrazyl.
The purpose and objectives of the study.
Study standardless method for assessing the antiradical activity of natural and synthetic antioxidants model 2,2-diphenyl-1-picrylhydrazyl using EPR spectroscopy. Testing methods for determining the anti-radical activity in the DPPH model EPR spectroscopy. Obtaining and analyzing anti-radical activity values for a number of substances with antiradical properties. Comparison with the results obtained by potentiometric determination of antioxidant activity using a mediator system.
Results. The optimum conditions for analysis. The research - the new reference-free method of evaluating anti-radical activity, a number of natural and synthetic antioxidants. Established values antiradical activity of natural and synthetic antioxidants. Various embodiments of the mechanisms of inhibition of free radicals and their antioxidant dependency structure.
奖励日期2016
源语言Russian
奖励机构
  • Ural Federal University
导师Алла Владимировна Иванова (Supervisor)

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