Поиск и отбор генотипов растений рода Alissum на устойчивость к никелю, меди и цинку
: магистерская диссертация

学生论文: Master's Thesis


The purpose of the work is to select plant genotypes of the genus Alissum that are resistant to increased concentrations of nickel, copper and zinc ions. Three species of plants from the genus Alissum sp. Brassicaceae family, namely: A. litvinovii, A. tortuosum, A. obovatum. During the experiment, plants were obtained from seeds, which were subjected to microclonal propagation. Plants were cultivated on media containing Cu2+, Ni2+ or Zn2 + at a concentration of 50 – 100 – 200 µM/L. Morphological measurements and descriptions were performed over 6 weeks. After the experiment, the content of Ni2+ ions in the raw biomass was determined using the Chugaev reagent. Plant genotypes that are resistant to an excess of heavy metals in the medium and capable of active growth under stress were selected; A. tortuosum genotype № 6 exceeded the control by 2.6 times and reached a size of 46.7 mm (200 µM Zn2+). Plants showed an accumulating ability with respect to nickel ions in vitro. Genotype № 5 A. obovatum accumulates 1.59 mg/g Ni2+ wet weight, which is 8 times higher than the control (200 µM Ni2+). This study confirms the promise of using plants from the genus Alyssum for phytoremediation. The final qualifying work of the master is 61 pages, 21 figures, 5 tables, 81 literary sources, of which 50 are Russian-speaking and 31 are English-speaking.
  • Ural Federal University
导师Александр Анатольевич Ермошин (Supervisor)